Güncel Patoloji Dergisi 2017, Cilt 1, Sayı 2
Diagnostic Significance of DOG-1 and CD117 Expressions in Testicular Germ Cell Tumors
Duygu GÜREL 1, Mehtat ÜNLÜ 1, Güven ASLAN 2, Uğur MUNGAN 2, Kutsal YÖRÜKOĞLU 1, Burçin TUNA 1
1 Dokuz Eylül Üniversitesi Tıp Fakültesi, Patoloji Anabilim Dalı, İZMİR
2 Dokuz Eylül Üniversitesi Tıp Fakültesi, Üroloji Anabilim Dalı, İZMİR
DOI : 10.5146/jcpath.2017.06 Aim: The cDNA microarray gene profile of gastrointestinal stromal tumors revealed that DOG1 gene (TMEM16A) was mostly expressed in these neoplasms. Immunohistochemically, DOG1 protein was found in a significant proportion of gastrointestinal stromal tumors. DOG-1 protein expression is generally found in CD117 positive tumors, and is also reported in melanoma and some carcinomas. However, testicular germ cell expression of DOG1 is not yet completely studied.

Materials and Methods: This study intended to identify DOG1 protein expression in seminomatous and non-seminomatous testicular germ cell tumors comparing with CD117 expression, using an anti-DOG1 polyclonal serum. Fifty cases including 26 mixed germ cell tumors, 15 seminomas, 6 embryonal carcinomas, 2 choriocarcinomas, and 1 teratoma were included in the study.

Results: CD117 expression was detected in 36 cases (14 seminomas, 20 mixed germ cell tumors, 1 teratoma and 1 embryonal carcinoma), and DOG-1 was detected in 4 cases (3 mixt germ cell tumors, 1 embryonal carcinoma). Except for an embryonal carcinoma, CD117 positivity was observed in seminomas (93%) and seminomatous component of mixed germ cell tumors. However, DOG-1 was positive in only 4 cases (3 mixt germ cell and 1 embryonal carcinoma). On the other hand, DOG-1 expression was found to be confined to the embryonal carcinoma component of MGHTs and embryonal carcinoma.Conclusion: These results show that DOG1 has no role in distinguishing between seminomatous and nonseminomatous tumors in testicular germ cell tumors. However, in retroperitoneal tumors with CD117 expression, DOG-1 appears to be an important biomarker in discriminating gastrointestinal stromal tumors from seminoma. Anahtar Kelimeler : Testis, Germ cell tumor, CD117, DOG-1, Differential diagnosis