2 Bahçelievler Memorial Hospital, Patoloji Bölümü, İSTANBUL
DOI : 10.5146/jcpath.2019.58 Aim: We compared the efficiencies achieved with the single and double staining methods of IHC antibodies used in daily practice for differential diagnosis in NSCLC biopsies not displaying a squamous or glandular differentiation. Less tissue was used with double antibody staining and it was verified whether it was possible to achieve tissue saving for the molecular tests to be applied where necessary.
Materials and Methods: Forty less differentiated and 8 well differentiated NSCLC cases identified as the control group were included into the scope of the study. Less differentiated cases were evaluated with IHC via TTF-1, p63, CK7 and CK5/6 antibodies with the single and double staining methods.
Results: Only 87.5% and 70% of the cases could be subtyped with the single staining method and double staining method. The false-negativity rates resulted to be high with the double staining method with the nucleus antibodies (TTF-1, p63) and that the false-negativity rates resulted to be high with the cytoplasmic antibodies (CK7, CK5/6). A change in diagnosis was observed in 14 cases (35%) in the comparison of the diagnoses achieved as a result of double and single staining methods.
Conclusion: In terms of saving tissues and procedures in double or multiple staining procedures, the results obtained with the method utilized in this study are not satisfactory. Our evaluation demonstrates that the reliability of the use of multiple antibodies to be put into practice without making a comparison according to the results obtained from single antibody procedures will be debatable.
Anahtar Kelimeler : Lung cancer, Non-small cell, Double staining, İmmunohistochemistry