Güncel Patoloji Dergisi 2019, Cilt 3, Sayı 2
Evaluation of Epstein Barr Virus in Diffuse Large B Cell Lymphomas by Using Chromogenic in Situ Hybridisation Technique
Aytül BUĞRA 1, Fetin Rüştü YILDIZ 2, Selver ÖZEKINCI 2, Gizem AYAZ 3, Zeynep Betül ERDEM 2, Çağlar ÇAKIR 2
1 Adalet Bakanlığı, İstanbul Adli Tıp Kurumu Başkanlığı, Histopatoloji Şubesi, İSTANBUL
2 Sağlık Bilimleri Üniversitesi, Okmeydanı Eğitim ve Araştırma Hastanesi, Patoloji Bölümü, İSTANBUL
3 Siirt Devlet Hastanesi, Patoloji Bölümü, SIIRT
DOI : 10.5146/jcpath.2019.50 Aim: Diffuse Large B Cell Lymphoma (DLBCL) is a Non-Hodgkin lymphoma. The etiology is unknown. EBER (EBV RNA) presence is seen between 8-11% in DLBCL; have been found to be associated with more advanced age, more advanced disease, and anaplastic and plasmablastic subtypes of DLBCL. With the revision of the World Health Organization (WHO) of lymphoma classification in 2016; as EBV (+) DLBCL begins to appear in younger and in wider spectrum, the resulting new information has led to the change of "elderly" to "not otherwise specified (NOS)". The aim of this study is to demonstrate the immunophenotypic properties of DLBCL and to show whether the presence of EBER detected by chromogenic in situ hybridization (CISH) in these cases is related to the age and sex of the cases.

Materials and Methods: The pathology reports of 61 cases diagnosed as DLBCL between January 2015 and December 2016 from the Okmeydanı Education and Research Hospital, Medical Pathology Department of Health Sciences University were examined. All hematoxylin and eosin (H & E) stained, bcl-2, bcl-6 and c-myc immunohistochemically stained preparations and EBER ISH preparations were reevaluated. The relationship between EBER positivity and age, sex, bcl-2, bcl-6, c-myc staining percentages were investigated.

Results: EBER positivity was determined in four cases of DLBCL cases. The ages of these cases ranged from 34 to 78 years. Staining was observed in 3 cases under 50 years of age (mean age is 43,6) and 1 case in over 50 years. 3 cases of EBER positive cases were male and 1 female. Positive staining was observed in 85,3% (n=52) with bcl-2, 95,1% (n=58) with Bcl-6 and 90,2% with c-myc (n=55) in varying percentages. There was no statistically significant relationship between EBER positivity and age, gender, bcl-2, bcl-6 and c-myc expression.

Conclusion: 6.6% EBER positive cases were detected in the DLBCL cases studied. More positivity was seen in males than females. The mean age of EBV positive DLBCLs is similar to the data in the literature. We believe that new studies are needed to investigate the presence of EBER not only in elderly patients but also in all age groups of DLBCL, both in relation to immunophenotypic findings and in order to define the characteristics of this newly defined group. Anahtar Kelimeler : DLBCL, EBER, Age, Bcl-2, Bcl-6, C-myc